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BIOCHEMISTRY II

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Learning Outcomes

  • Will be able to explain general information about carbohydrates
  • Classifies carbohydrates.
  • Explains the stereoisomeric structures of carbohydrates
  • Explains the derivatives of carbohydrates.
  • Draws the linear and cyclic chemical formulas of common monosaccharides
  • Knows and names the formation of glycosidic bonds.
  • Draws the chemical formulas of common disaccharides
  • Explains the general properties of common disaccharides
  • Explains the general formulas of common polysaccharides
  • Explains the general properties of common polysaccharides.
  • Explains the functions of compound carbohydrates.
  • Will be able to explain the digestion and absorption of carbohydrates
  • Explains the digestion of carbohydrates.
  • Explains the properties of enzymes involved in digestion
  • Explains the passage of monosaccharides through the intestinal epithelial cells
  • Will be able to explain glycolysis
  • Explains the uptake of glucose into cells
  • Explains the purpose of glycolysis.
  • Writes the reactions of glycolysis
  • Explains the pathways that feed into glycolysis
  • Explains the utilization of pyruvate under anaerobic conditions
  • Explains how glycolysis is regulated.
  • Will be able to explain gluconeogenesis
  • Explains the necessity of gluconeogenesis.
  • Writes the reactions of gluconeogenesis
  • Writes the energy utilization steps in gluconeogenesis
  • Explains how glycolysis and gluconeogenesis are reciprocally regulated
  • Will be able to explain glycogen metabolism
  • Explains the synthesis reactions of glycogen
  • Explains the degradation reactions of glycogen
  • Explains how the synthesis and degradation reactions of glycogen are reciprocally regulated
  • Explains the hormonal regulation of glycogen synthesis and degradation reactions
  • Will be able to explain the pentose phosphate pathway in glucose oxidation
  • Explains the purpose of the pentose phosphate pathway.
  • Explains the oxidative phase of the pentose phosphate pathway.
  • Explains the non-oxidative phase of the pentose phosphate pathway.
  • Explains the relationship between the products of the pentose phosphate pathway and other metabolic pathways
  • Explains the relationship between the pentose phosphate pathway and glycolysis
  • Will be able to explain the citric acid cycle
  • Lists the products that pyruvate is converted into.
  • Explains the reactions involved in the production of Acetyl-CoA
  • Writes the reactions of the citric acid cycle.
  • Explains the energy gain obtained from the citric acid cycle reactions.
  • Explains how the citric acid cycle is regulated.
  • Will be able to explain general information about lipids.
  • Explains the properties of lipids and fatty acids
  • Draws the chemical structure of lipids
  • Classifies lipids
  • Explains the structural properties of lipid subclasses
  • Explains the functions of essential fatty acids
  • Draws the structure of triacylglycerols.
  • Explains the structures of structural lipids in membranes
  • Explains the structure of cholesterol.
  • Explains the steroid structures derived from cholesterol
  • Will be able to explain lipid biosynthesis.
  • Explains the biosynthesis of fatty acids.
  • Explains the biosynthesis of triacylglycerols
  • Explains the biosynthesis of membrane phospholipids
  • Explains the biosynthesis of cholesterol.
  • Will be able to explain the oxidation of lipids.
  • Explains the digestion of dietary lipids
  • Explains the functions of bile acids.
  • Explains the formation of primary and secondary bile acids
  • Explains the absorption of dietary lipids in the small intestine
  • Explains the functions of lipoproteins
  • Explains the structures of lipoproteins.
  • Explains the metabolism of lipoproteins
  • Explains the types of lipid oxidation
  • Writes the steps of β-oxidation
  • Explains how the reactions of β-oxidation are regulated.
  • Explains the stages of ketone body production.
  • Will be able to explain the integration of metabolism
  • Explains the metabolism in the fasting state
  • Explains the metabolism in the fed state.
  • Explains the relationships between different metabolic pathways
  • Will be able to explain the electron transport chain.
  • Explains the pathways of electron transfer to the mitochondria.
  • Explains the function of enzymes involved in biological oxidation reactions.
  • Explains the structure of complexes involved in the electron transport chain
  • Explains electron transfer between the complexes in the electron transport chain
  • Explains the functions of electron transport chain inhibitors.
  • Will be able to explain oxidative phosphorylation.
  • Establishes the relationship between the electron transport chain and oxidative phosphorylation
  • Explains the chemiosmotic theory
  • Explains ATP synthesis
  • Explains how oxidative phosphorylation is regulated
  • Will be able to explain vitamins.
  • Explains the biological significance
  • Classifies vitamins
  • Explains the structure and functions of fat-soluble vitamins
  • Explains the structure and functions of water-soluble vitamins.
  • Explains vitamin-like compounds
  • Will be able to explain minerals.
  • Explains the functions of minerals involved in biological systems
  • Explains their biochemical significance.
  • Will be able to explain the endocrine system.
  • Defines the endocrine system
  • Explains the mechanisms of hormone action.
  • Explains the classification of hormones
  • Will be able to explain thyroid hormones.
  • Explains the biosynthesis of thyroid hormones
  • Explains the regulation of thyroid hormone synthesis and release.
  • Explains the transport and metabolism of thyroid hormones.
  • Explains the metabolic effects of thyroid hormones.
  • Explains the effects of thyroid hormones on carbohydrate metabolism
  • Explains the effects of thyroid hormones on fat metabolism
  • Explains the effects of thyroid hormones on protein metabolism
  • Explains the effects of thyroid hormones on growth
  • Will be able to explain the hormones that regulate calcium and phosphorus metabolism.
  • Explains the structure and biosynthesis of parathyroid hormone.
  • Explains the biological effects of parathyroid hormone
  • Explains the regulation of parathyroid hormone release.
  • Explains the structure of calcitonin
  • Explains the biological effects of calcitonin
  • Explains the regulation of calcitonin release
  • Explains the structure and biosynthesis of calcitriol
  • Explains the regulation of calcitriol synthesis and metabolism
  • Explains the biological effects of calcitriol
  • Will be able to explain pancreatic hormones.
  • Explains the structure, biosynthesis, and secretion of insulin
  • Explains the effects of insulin on carbohydrate metabolism.
  • Explains the effects of insulin on lipid metabolism
  • Explains the effects of insulin on protein and nucleic acid metabolism.
  • Explains the breakdown of insulin
  • Explains the chemical structure of glucagon
  • Explains the regulation of glucagon release
  • Explains the biological effects of glucagon
  • Explains the general characteristics of somatostatin
  • Explains the general characteristics of pancreatic polypeptide
  • Will be able to explain adrenal hormones.
  • Explains the biosynthesis of adrenal medulla hormones
  • Explains the storage and release of adrenal medulla hormones.
  • Explains the metabolism of catecholamines.
  • Explains the metabolic effects of catecholamines.
  • Explains the biosynthesis of adrenal corticosteroids.
  • Explains the transport of adrenal steroids.
  • Explains the metabolism and release of steroid hormones.
  • Explains the biological effects of corticosteroids.
  • Will be able to explain gonads and steroid hormones.
  • Explains the biosynthesis of androgens
  • Explains the transport and metabolism of testosterone
  • Explains the formation of active hormones derived from testosterone
  • Explains the biological effects of androgens
  • Explains the biosynthesis of estrogens
  • Explains the transport of estrogen and progesterone
  • Explains the metabolism of estrogens.
  • Explains the biological effects of estrogens
  • Explains the biosynthesis and metabolism of progesterone
  • Explains the biological effects of progesterone
  • Will be able to explain hypothalamic and pituitary hormones.
  • Explains the biological effects of Thyrotropin-releasing hormone.
  • Explains the biological effects of Corticotropin-releasing hormone
  • Explains the biological effects of Growth hormone-releasing hormone
  • Explains the biological effects of Somatostatin
  • Explains the biological effects of Gonadotropin-releasing hormone.
  • Explains the biological effects of Prolactin-releasing and inhibiting hormones
  • Explains the biological effects of Adrenocorticotropic hormone.
  • Explains the biological effects of Melanocyte-stimulating hormone
  • Explains the biological effects of Gonadotropins.
  • Explains the regulation of synthesis and release of Gonadotropins.
  • Explains the biological effects of Thyroid-stimulating hormone
  • Explains the structure and biological effects of Somatotropin hormone
  • Explains the regulation of Somatotropin secretion
  • Explains the structure and biological effects of Prolactin hormone.
  • Explains the regulation of Prolactin secretion
  • Explains the biological effects of Antidiuretic hormone
  • Explains the biological effects of Oxytocin
  • Will be able to explain gastrointestinal system hormones.
  • Lists the gastrointestinal hormones.
  • Lists the cellular locations of gastrointestinal hormones.
  • Lists the main effects of gastrointestinal hormones
  • Will be able to explain analysis examples
  • Explains the chemical composition of blood and the samples obtained from blood
  • Explains how to obtain the desired blood sample.
  • Explains hemolysis and its effect on biochemical parameters
  • Explains the important considerations when preparing blood samples
  • Describes the characteristics of the tubes used for blood collection.
  • Classifies the compounds contained in serum
  • explains the content of cerebrospinal fluid (CSF).
  • Explains the reasons for using CSF in biochemical analyses.
  • Defines paracentesis fluids
  • Lists the characteristics of paracentesis fluids
  • Lists the similarities or differences between paracentesis fluids
  • Explains the method used to separate paracentesis fluids
  • Explains the reasons for using paracentesis fluids in biochemical analyses
  • Explains the content of residuum
  • Defines residuum.
  • Explains the reasons for using residuum in biochemical analyses
  • Explains the content of synovial fluid.
  • Explains the reasons for using synovial fluid in biochemical analyses
  • Explains the content of amniotic fluid.
  • Explains the reasons for using amniotic fluid in biochemical analyses
  • Explains the physical and chemical properties of stool.
  • Explains the reasons for using stool in biochemical analyses
  • Explains the methods used in the examination of biological materials.
  • Will be able to explain the main anticoagulant substances
  • Lists anticoagulant substances.
  • Explains the structure of anticoagulant substances.
  • Explains the procedure for using anticoagulant substances.
  • Explains the interaction between analysis and the selection of anticoagulant substances
  • Will be able to explain protein recognition reactions
  • Recognizes the appropriate sample.
  • Lists the methods used in recognition reactions
  • Explains the characteristics of the methods used in analysis.
  • Explains the principle of the analytical method.
  • Draws the molecular structure of the reagent
  • Explains how the analytical method is applied.
  • Explains the purpose of the chemicals used in the analysis.
  • Recognizes the materials and devices used in the analytical method
  • Explains the possible causes of the analytical result.
  • Recognizes the color of the compound formed as a result of the analysis and draws its chemical structure.
  • Recognizes the appropriate sample
  • Explains the Biuret method
  • Lists the methods used in analysis
  • Explains the characteristics of the methods used in analysis
  • Explains the principle of the analytical method
  • Explains how the analytical method is applied.
  • Explains the purpose of the chemicals used in the analysis.
  • Recognizes the materials and devices used in the analytical method
  • Explains the possible causes of the analytical result.
  • Recognizes the appropriate sample.
  • Will be able to explain the Bromcresol Green method
  • Explains the principle of the analytical method
  • Explains how the analytical method is applied.
  • Explains the purpose of the chemicals used in the analysis.
  • Recognizes the materials and devices used in the analytical method.
  • Explains the possible causes of the analytical result.
  • Explains how the globulin amount is determined.
  • Will be able to explain the denaturation of proteins
  • Explains the definition of denaturation.
  • Lists the factors that cause denaturation.
  • Lists the chemicals used in denaturation.
  • Explains the purpose of the chemicals used in the denaturation process.
  • Explains how the denaturation process is applied.
  • Explains the results of the denaturation process.
  • Explains protein precipitation methods.
  • Will be able to explain deproteinization
  • Explains the definition of deproteinization.
  • Explains the purpose of the process.
  • Lists the important considerations in the process.
  • Lists the methods used in deproteinization.
  • Explains the methods used in deproteinization.
  • Explains how the deproteinization process is applied.
  • Explains the concept of isoelectric point.
  • Explains the concept of salting out.
  • Explains the principle of the experiment.
  • Will be able to explain the Ninhydrin test.
  • Explains how the experiment is applied.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains the possible results of the experiment.
  • Lists the amino acids that react with ninhydrin.
  • Explains the principle of the experiment.
  • Will be able to explain the Xanthoprotein reaction.
  • Explains how the experiment is applied.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains the possible results of the experiment.
  • Lists the amino acids that react with xanthoprotein.
  • Explains the principle of the experiment.
  • Will be able to explain the Sulfur test.
  • Explains how the experiment is applied.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains the possible results of the experiment.
  • Lists the amino acids that give a positive result in the sulfur test.
  • Will be able to explain the tests that measure renal blood flow.
  • List the renal function tests.
  • Explain the renal function tests.
  • List the concentration tests.
  • List the dilution tests.
  • Explain the substances used in renal function tests.
  • Explain the reasons for choosing creatinine as a biochemical parameter.
  • Explain how the creatinine clearance test is performed.
  • Explain the formula used in clearance calculation.
  • Define the reference range for creatinine clearance values.
  • List the samples used in the creatinine quantification analysis.
  • Explain the purpose of the creatinine quantification analysis.
  • State the method used for creatinine quantification.
  • Explain the principle of the creatinine quantification method.
  • Explain how the creatinine quantification method is performed.
  • Explain the purpose of the chemicals used in the creatinine quantification method.
  • Recognize the materials and devices used in the analysis method.
  • Explain the creatinine values in samples from healthy individuals.
  • Will be able to explain the definition and classification of function tests.
  • Explain the purpose of liver function tests.
  • Classify liver function tests.
  • Explain the tests related to carbohydrate metabolism.
  • Explain the tests related to lipid metabolism.
  • Explain the tests related to protein metabolism.
  • List the tests related to protein metabolism.
  • Explain the tests related to detoxification.
  • Explain the tests related to bilirubin metabolism.
  • Explain the purpose of tests for the determination of serum enzymes.
  • List the tests that provide information about liver function.
  • Will be able to explain the determination of bilirubin levels.
  • Knows the name of the method.
  • Recognizes the materials and devices used in the analysis method.
  • Explains the principle of the analysis method.
  • Explains how the analysis method is performed.
  • Explains the purpose of the chemicals used in the analysis.
  • Explains the preparation of the diazo reagent.
  • Knows the serum bilirubin level values in healthy individuals.
  • Explains the precautions to be taken during the analysis.
  • Will be able to explain the Benedict test.
  • Explains the purpose of the experiment.
  • Explains the principle of the experiment method.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains how the experiment method is performed.
  • Explains the possible observation results of the experiment and their reasons.
  • Will be able to explain the Molisch test.
  • Explains the purpose of the experiment.
  • Explains the principle of the experiment method.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains how the experiment method is performed.
  • Explains the possible observation results of the experiment and their reasons.
  • Will be able to explain the Iodine test.
  • Explains the purpose of the experiment.
  • Explains the principle of the experiment method.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains the reason for the interaction between iodine and starch.
  • Explains how the experiment method is performed.
  • Explains the possible observation results of the experiment and their reasons.
  • Will be able to explain the Osazone test.
  • Explains the purpose of the experiment.
  • Explains the principle of the experiment method.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains how the experiment method is performed.
  • Explains the possible results of the experiment.
  • Explains the possible observation results of the experiment and their reasons.
  • Will be able to explain the Hydrolysis test.
  • Explains the purpose of the experiment.
  • Explains the principle of the experiment method.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains how the experiment method is performed.
  • Explains the possible observation results of the experiment and their reasons.
  • Will be able to explain other carbohydrate identification tests.
  • Lists other carbohydrate identification tests.
  • Explains the purpose of the experiment.
  • Explains the principle of other carbohydrate identification tests.
  • Explains how the experiment method is performed.
  • Explains the condition indicated by the experiment results.
  • Will be able to explain non-enzymatic methods.
  • Explains the sample used in the analysis.
  • Recognizes the materials and devices used in the analysis method.
  • Lists the analysis methods.
  • Explains the principle of the analysis methods.
  • Explains the precautions to be taken during the analysis.
  • Will be able to explain enzymatic methods.
  • Explains the sample used in the analysis.
  • Recognizes the materials and devices used in the analysis method.
  • Lists the analysis methods.
  • Explains the principle of the analysis methods.
  • Explains the method with a chemical reaction.
  • Explains the precautions to be taken during the analysis.
  • Knows the reference range of blood glucose concentration in healthy individuals.
  • Will be able to explain the dehydration of glycerol.
  • Explains the purpose of the experiment.
  • Explains the principle of the experiment method.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains how the experiment method is performed.
  • Explains the possible observation results of the experiment and their reasons.
  • Will be able to explain the binding of iodine to oleic acid.
  • Explains the principle of the experiment method.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains how the experiment method is performed.
  • Explains the possible observation results of the experiment and their reasons.
  • Knows the name of the method.
  • Will be able to explain the determination of total cholesterol levels.
  • Explains the purpose of the experiment.
  • Explains the principle of the experiment method.
  • Explains the purpose of the chemicals used in the experiment.
  • Explains how the experiment method is performed.
  • Explains the possible observation results of the experiment and their reasons.
  • Explains the precautions to be taken during the analysis.
  • Knows the reference range of blood cholesterol concentration in healthy individuals.
  • Explains the working principle of the test.
  • Will be able to explain the determination of β-hCG in urine.
  • Explains the reason for selecting β-hCG as a diagnostic parameter in pregnancy.
  • Explains the possible observation results of the test and their reasons.
  • Explains the precautions to be taken during the test.
  • Explains the level of hCG in the blood of a non-pregnant woman.
  • Will be able to explain the biochemical analysis of urine.
  • Classifies urine samples.
  • Lists the physical properties of urine.
  • Explains the physical properties of urine.
  • Defines changes in urine volume.
  • Explains the pathological conditions indicated by the color of urine.
  • Defines the changes observed in the appearance of urine.
  • Explains the changes observed in the appearance of urine.
  • Explains the methods for determining urine density.
  • Lists the instruments used to measure urine density.
  • Defines the changes detected in urine density.
  • Explains the changes detected in urine density.
  • Knows the reference values for urine density.
  • Explains the concept of urine osmolality.
  • Recognizes the instrument used to measure urine osmolality.
  • Explains the changes observed in the odor of urine.
  • Explains the chemical properties of urine.
  • Explains the method used for detecting glucose in urine.
  • Explains the presence of lactose in urine.
  • Explains the presence of bilirubin in urine.
  • Explains the method used for determining bilirubin in urine.
  • Explains the presence of urobilinogen in urine.
  • Explains the method used for determining urobilinogen in urine.
  • Explains the presence of acetone in urine.
  • Explains the method used for determining acetone in urine.
  • Will be able to explain the microscopic analysis of urine.
  • Explains the preparation of the urine sample for analysis.
  • Explains the cells seen after microscopic examination.
  • Explains the casts seen after microscopic examination.
  • Explains the presence of bacteria in urine.
  • Lists the bacteria found in urine.
  • Explains the presence of parasites in urine.
  • Lists the parasites found in urine.
  • Classifies the crystals observed in urine.
  • Explains the physical properties of the crystals observed in urine.
  • Will be able to explain urine test strips.
  • Explains the working principle of urine test strips.
  • Explains the preparation of the urine sample for testing.
  • Lists the parameters that can be measured with test strips.
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